Manuel Nucleic acid isolation

Analytik Jena offers an extraordinary wide range of purification and isolation kits for nucleic acids, all made in Germany. Those are based on own technologies and patents. It ranges from manual to automated solutions for DNA/RNA extraction of most starting materials.

 

bs_Imagbild_Kits

For fast and efficient purification of PCR products from PCR reaction mixtures based on a new two-step-procedure (bind and elute). No washing steps are necessary. Using 96 well filterplates in combination with a centrifuge the kit allows the purification of up to 96 samples parallel. The whole procedure is ready within 30 minutes.

 

Storage conditions:
The innuPREP PCRpure 96 Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Binding Buffer, Elution Buffer, Binding Plate, Deep Well Plate, Elution Plate, Sealing Foil, Manual

Order-No.
 Amount
845-FP-5010192 2 x 96 reactions
845-FP-5010384 4 x 96 reactions
845-FP-5010960 10 x 96 reactions
 

For fast and efficient purification of PCR products from PCR reaction mixtures based on a new two-step-procedure (bind and elute). No washing steps are necessary. The whole isolation is finished within 3 minutes, only.

 

Product specifications:

1. Starting material:
PCR reaction mixtures (up to 50 µl)

2. Time for isolation:
3 minutes
Based on a new two-step procedure

3. Typical yield:
60 bp – 30 kbp

4. Rate of recovery:
75 – 95%
Depending on the length of the PCR fragments

Storage conditions:
The innuPREP PCRpure Kit should be stored dry, at room temperature (14°C – 25°C). It is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Binding Buffer, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5010010 10 reactions
845-KS-5010050 50 reactions
845-KS-5010250 250 reactions
 

For extraction and recovery of DNA fragments from TAE or TBE agarose gels.

 

Product specifications:

1. Starting material:

  • TAE agarose gels (up to 300 mg)
  • TBE agarose gels (up to 300 mg)

2. Time for isolation:
Approximately 20 minutes

3. Fragment length:
100 bp – 30 kb

4. Rate of recovery:
65 – 90 %
Depending on the length of DNA amplicons

Storage conditions:
The innuPREP Gel Extraction Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Gel Solubilizer, Binding Optimizer, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5030010 10 reactions
845-KS-5030050 50 reactions
845-KS-5030250 250 reactions
 

For fast and efficient purification of PCR products from PCR reaction mixtures based on a new two-step-procedure and/or for the extraction and recovery of DNA fragments from TAE or TBE agarose gels.

 

Product specifications:

1. Starting material:
TAE or TBE agarose gels (up to 300 mg)

2. Time for isolation:
Approximately 20 minutes

3. Fragment length:
100 bp – 30 kbp

4. Rate of recovery:
65 – 90% (depending on the length of DNA amplicons)

Storage conditions:
The innuPREP DOUBLEpure Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Gel Solubilizer, Binding Optimizer, Binding Buffer, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5050010 10 reactions
845-KS-5050050 50 reactions
845-KS-5050250 250 reactions
 

For fast removing of dye terminators from sequencing mixes based on a new two-stepprocedure (bind and elute). No washing steps are necessary. No ethanol precipitation or sephadex.

 

Storage conditions:
The innuPREP DYEpure Kit should be stored dry, at room temperature (14°C – 25°C). It is stable for at least 12 months under these conditions.
Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
DYE Removal Buffer, Molecular biology grade H2O, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5020010 10 reactions
845-KS-5020050 50 reactions
845-KS-5020250 250 reactions
 

For isolation of plasmid DNA from 1 – 5 ml bacteria suspension.

 

Product specifications:

1. Starting material
Bacteria suspension (0.5 – 5.0 ml)

2. Time for isolation
Approximately 16 minutes

3. Typical yield
Approximately 25 µg
Typical yield from 2.0 ml starting material: 6 – 14 µg

Storage conditions:
The innuPREP Plasmid Mini Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

 

Order-No.
 Amount
845-KS-5040010 10 reactions
845-KS-5040050 50 reactions
845-KS-5040250 250 reactions
 

For isolation of high yield plasmid DNA from 5 – 15 ml bacteria suspension. The kit uses the fast and effective Spin Filter mini format to isolate up to 40 µg of high-quality plasmid DNA.

 

Product specifications:

1. Starting material
Bacteria suspension (5.0 – 15.0 ml)

2. Time for isolation
Approximately 20 minutes

3. Typical yield
Approximately 30 - 40 µg

Storage conditions:
The innuPREP Plasmid Mini Kit Plus should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5240010 10 reactions
845-KS-5240050 50 reactions
845-KS-5240250 250 reactions
 

For the fast and direct isolation of plasmid DNA from 250 µl of a bacteria suspension. The kit doesn‘t need the pelleting of bacteria cells and starts directly with the bacterial suspension. The kit allows the isolation of 1 – 4 µg plasmid DNA.

 

Product specifications:

1. Starting material:
Bacteria suspension (0.5 – 5.0 ml)

2. Time for isolation
Approximately 6 minutes

3. Typical yield
Approximately 25 µg
Typical yield from 2.0 ml starting material: 6 – 14 µg

Storage conditions:
The innuPREP Plasmid Rapid Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Resuspension Buffer, Lysis Buffer, Washing Solutions, Elution Buffer, Pre-Filter, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-5140010 10 reactions
845-KS-5140050 50 reactions
845-KS-5140250 250 reactions
 

For the fast and direct isolation of plasmid DNA from 250 µl of a bacteria suspension. The kit doesn‘t need the pelleting of bacteria cells and starts directly with the bacterial suspension. The kit allows the isolation of 1 – 4 µg plasmid DNA.

 

Product specifications:

1. Starting material
Bacteria suspension (250 µl, direct)

2. Time for isolation
Approximately 12 minutes

3. Typical yield
Approximately 1 – 3 µg from 200 µl starting material (high copy plasmid)

Storage conditions:
The innuPREP Plasmid Small Kit should be stored dry, at room temperature (14°C - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Buffer, Neutralization Buffer, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Manual

Order-No.
 Amount
845-KS-5340010 10 reactions
845-KS-5340050 50 reactions
845-KS-5340250 250 reactions
 

For very fast and efficient isolation of genomic DNA from small amounts of different types of starting material like microbiopsies, whole blood up to 50 µl, blood sticks and from limited amounts of cells.

 

Product specifications:

1. Starting material:

  • Small pieces of tissue samples (up to 5 mg)
  • Paraffine embedded tissue samples
  • Eucaryotic cells (up to 1 x 106 cells)
  • Whole blood samples (up to 50 µl) and blood sticks   

2. Time for isolation:   
Approximately 8 minutes after lysis step   

3. Typical yield:
Depends on type and amount of starting material
Binding capacity of the spin column is > 100 µg gDNA

4. Typical ratio
A260:A280: 1.7 – 2.0  
The extracted gDNA can be used for a wide range of different molecular biology applications.

Storage conditions:
The innuPREP DNA Micro Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C and dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1010010 10 reactions
845-KS-1010050 50 reactions
845-KS-1010250 250 reactions
 

For isolation of genomic DNA from rodent tails. The protocol has been specially optimized to get a maximum yield and quality of genomic DNA from rodent tails.

 

Production specifications:

1. Starting material

  • Rodent tails
  • Mouse tail max. 0.5 – 1.2 cm
  • Rat tail max. 0.2 – 0.6 cm

2. Time for isolation
Lysis: 1 – max. 3 hours
Extraction: approx. 9 min

3.Typical quality and yield
Mouse tail (1.2 cm): 30 – 40 µg
Rat tail (0.6 cm): 35 – 45 µg

Ratio A260:A280: 1.8 – 2.0

Storage conditions:
The blackPREP Rodent Tail DNA Kit should be stored dry, at room temperature
(14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Manual

Order-No.
 Amount
845-BP-0010010 10 reactions
845-BP-0010050 50 reactions
845-BP-0010250 250 reactions
 

For isolation of genomic DNA up to 100 mg of different kinds of plant material. The kit combines the lysis of starting material, filtration of unlysed components and subsequent binding of genomic DNA on a Spin Filter surface. After washing the bound DNA will be eluted from the Spin Filter.

 

Product specifications:

1. Starting material:
Fresh, frozen or dried plant material (up to 100 mg)

2. Time for isolation:
Approximately 30 – 40 minutes

3. Typical yield:
3 – 25 µg
Depending on the kind and initial amount of the starting material

Storage conditions:
The innuPREP Plant DNA Kit should be stored dry, at room temperature (14°C–25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Pre-Filter, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1060010 10 reactions
845-KS-1060050 50 reactions
845-KS-1060250 250 reactions
 

For isolation of genomic DNA from whole blood samples from 0.5 ml up to 5 ml using simple Spin Filter mini format. The kit combines the selective lysis of erythrocytes, pelleting of nucleus containing blood cells. After red blood cell lysis the collected lymphocytes will be transfered into a Lysis Tube PLP. The Lysis Tube PLP contains solid formula of proteolytic reagents. No reagents have to be added for the lysis step. After lysis of cells proteins will be removed by selective precipitation following binding of the genomic DNA on a Spin Filter
surface and elution of the bound DNA. Because of the mini Spin Filter format the whole procedure is very fast and efficient and takes place in a standard table centrifuge. The kit allows the isolation of more than 100 µg genomic DNA.

 

Product specifications:

1. Starting material:

  • Fresh or frozen whole blood (0.5 – 5.0 ml)
  • Stabilized with EDTA or citrate

2. Time for isolation:
Approximately 30 – 40 minutes

3. Typical yield:
10 – 100 µg
Depending on initial volume of whole blood

4. Binding capacity:
Approx.: 100 µg

Storage conditions:
The innuPREP Master Blood Kit should be stored dry, at room temperature
(14° - 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Ery Lysis Solution, Lysis Tube, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1070010 10 reactions
845-KS-1070050 50 reactions
845-KS-1070250 250 reactions
 

For isolation of genomic DNA from whole blood samples from 0.5 ml up to 2.0 ml. The kit combines the selective lysis of erythrocytes, pelleting of nucleus containing blood cells and subsequent lysis of the cells and protein removing following binding of the genomic DNA on a Spin Filter surface and elution of the bound DNA.

 

Product specifications:

1. Starting material:

  • Fresh, frozen or whole blood (0.5 – 2 ml)
  • Stabilized with EDTA or citrate

2. Time for isolation:
Approximately 30 - 40 minutes

3. Typical yield:
10 – 50 µg
Depending on initial volume of whole blood

4. Binding capacity:
Approximately 100 µg    

Storage conditions:
The innuPREP Blood DNA Midi Kit should be stored dry, at room temperature (14°C–25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Ery Lysis Solution, Lysis Solution, Precipitation Buffer, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1030010 10 reactions
845-KS-1030050 50 reactions
845-KS-1030250 250 reactions
 

For isolation of genomic DNA direct from whole blood samples up to 300 µl.

 

Product specifications:

1. Starting material:

  • Whole blood (300 µl)

2. Time for isolation:
Approximately 20 minutes

3. Typical yield:
3 – 8 µg/250µl whole blood

Storage conditions:
The innuPREP Blood DNA Mini Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, elution Tubes, Manual

Order-No.
 Amount
845-KS-1020010 10 reactions
845-KS-1020050 50 reactions
845-KS-1020250 250 reactions
 

For very fast and efficient isolation of genomic DNA from small amounts of different types of forensic samples like hairs or hair roots, stains of blood, saliva or sperm, finger nails, cigarette butts, bubble gum, buccal swabs, stamps and envelopes as well as fingerprints on different surfaces. The kit is a very efficient tool for isolation of DNA for applications where only limited amounts of starting material and very dirty material is available. Further, the new kind of extraction chemistry allows particularly the recovery of degraded DNA.

 

Storage conditions:
The innuPREP Forensic Kit should be stored dry, at room temperature (14°C–20 °C) and is stable for at least 6 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1050010 10 reactions
845-KS-1050050 50 reactions
845-KS-1050250 250 reactions
 

Every buccal swab is packed separately and sterile in a tube with an inscription field. It consist of wood and cotton. A buccal swab is the easiest way to collect samples of different origin and source and provides so an extensive possibility to get human and animal samples. The assay can be handled and transported easily and is protected against contamination.

 
Order-No. Amount
845-SW-1040100 100 pieces


Further information will follow soon.

 

For the very fast and efficient isolation of high yield genomic DNA from buccal swabs. The
recovery of DNA and the quality are excellent. The kit contains also swabs for sample
collecting.

 

Product specifications:

1. Starting material:
Buccal swabs

2. Time for isolation:
Approximately 15 minutes after lysis step

3. Typical yield:
Depends on type and amount of starting material
Binding capacity of the spin column is > 100 µg gDNA

4. Typical ratio
A260:A280:1.7 – 2.0

Storage conditions:
The innuPREP Swab DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Buccal Swabs, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Pre-Filter, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1080010 10 reactions
845-KS-1080050 50 reactions
845-KS-1080250 250 reactions
 

For very fast and efficient isolation of genomic DNA from different amounts and different
types of starting material like tissue samples up to 50 mg, paraffin embedded tissue mate-
rial, buccal swabs, mouse or rodent tail, eucaryotic cell pellets.

 

Product specicifations

1. Starting material:    

  • Tissue samples (up to 50 mg)
  • Rodent tail (up to 1 cm)
  • Paraffin embedded tissue samples
  • Buccal swabs
  • Eucaryotic cells (up to 5x106 cells)

2. Time for isolation:   
Approximately 15 minutes after lysis step   

3. Typical yield:   
Depends on type and amount of starting material
Binding capacity of the spin column is > 100 µg gDNA

4. Typical ratio

A260:A280 : 1.7 – 2.0   

Storage conditions:
The innuPREP DNA Mini Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-1040010 10 reactions
845-KS-1040050 50 reactions
845-KS-1040250 250 reactions
 

For very fast and efficient parallel isolation of genomic DNA and cellular total RNA from eucaryotic cells, bacteria and tissue samples.

 

Product specifications:

1. Starting material:

  • Eucaryotic cells (max. 5 x 106)
  • Tissue sample (up to 20 mg)
  • Bacterial cells (gram+ and gram- bacteria, up to 1 x 109)

2. Time for isolation:
Approximately 15 – 40 min

3. Typical yield:
Depending on the kind and initial amount of the starting material

4. Binding capacity:
Approximately: 100 µg RNA > 10 µg DNA

Storage conditions:
The innuPREP DNA/RNA Mini Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Washing Solutions, RNase-free Water, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-2080010 10 reactions
845-KS-2080050 50 reactions
845-KS-2080250 250 reactions
 

For very fast and efficient isolation of cellular total RNA from eucaryotic cells, bacteria and tissue samples.

 

Product specifications:

1. Starting material:

  • Eucaryrotic cells (5 x 106)
  • Tissue samples (up to 20 mg)
  • gram+ and gram- bacteria (1 x 109)
  • Biopsies
  • Paraffine embedded tissue samples

2. Time for isolation:
Approximately 15 - 40 minutes

3. Typical yield:
Not determined. The yield depends on the type and the amount of the starting material.

4. Binding capacity:
Approximately: 100 µg RNA

Storage conditions:
The innuPREP RNA Mini Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Washing Solution, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-2040010 10 reactions
845-KS-2040050 50 reactions
845-KS-2040250 250 reactions
 

For very fast and efficient isolation of cellular total RNA incl. isolation of small RNA molecules from eucaryotic cells, bacteria and tissue samples.

 

Product specifications:

1. Starting material:

  • Eucaryrotic cells (5 x 106)
  • Tissue samples (up to 20 mg)
  • gram+ and gram- bacteria (1 x 109)
  • Biopsies
  • Paraffine embedded tissue samples

2. Time for isolation:
Approximately 15 - 40 minutes

3. Typical yield:
Not determined. The yield depends on the type and the amount of the starting material.

4. Rate of recovery:
High rate of recovery for small RNA molecules.

5. Binding capacity:
Approximately: 100 µg RNA

Storage conditions:
The innuPREP Micro RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Washing Solution, Binding Solution, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-2030010 10 reactions
845-KS-2030050 50 reactions
845-KS-2030250 250 reactions
 

For very fast and efficient parallel isolation of genomic DNA and cellular total RNA from eucaryotic cells, bacteria and tissue samples.

 

Product specifications:

1. Starting material:

  • Eucaryotic cells (max. 5 x 106)
  • Tissue sample (up to 20 mg)
  • Bacterial cells (gram+ and gram- bacteria, up to 1 x 109)

2. Time for isolation:
Approximately 15 – 40 min

3. Typical yield:
Depending on the kind and initial amount of the starting material

4. Binding capacity:
Approximately: 100 µg RNA > 10 µg DNA

Storage conditions:
The innuPREP DNA/RNA Mini Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Washing Solutions, RNase-free Water, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-2080010 10 reactions
845-KS-2080050 50 reactions
845-KS-2080250 250 reactions
 

For very fast and efficient isolation of cellular total RNA from 0.5 ml – 1.0 ml of whole blood.

 

Product specifications:

1. Starting material:
Whole blood (0.5 – 1 ml)

2. Time for isolation:
Approximately 45 minutes

3. Typical yield:
1 – 8 µg Depending on the kind and initial volume of whole blood

Storage conditions:
The innuPREP Blood RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store Buffer ELB at 4°C.

Kit components:
Buffer ELB, Concentrate ELB, Lysis Solution, Washing Solution, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-2010010 10 reactions
845-KS-2010050 50 reactions
845-KS-2010250 25 reactions
 

For very fast and efficient isolation of cellular total RNA from different types of plant material.

 

Product specifications:

1. Starting material:
Different kinds of plant material (up to 100 mg)

2. Time for isolation:
Approximately 30 minutes after homogenization

3. Typical yield:
Depending on the kind and initial amount of the starting material

4. Binding capacity:
Approximately: 100 µg RNA

Storage conditions:
The innuPREP Plant RNA Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solutions, Washing Solutions, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-2060010 10 reactions
845-KS-2060050 50 reactions
845-KS-2060250 25 reactions
 

For isolation of DNA from pellets of gram+ and gram– bacteria after cultivation. The extraction procedure combines a lysis step with lysozyme following Proteinase K digestion and subsequent efficient binding of genomic DNA on a Spin Filter surface. The bound DNA will be washed and finally eluted.

 

Product specifications:

1. Starting material
Culture pellets of gram+ and gram- bacteria

2. Time for isolation
Approximately 15 minutes after the lysis step

3. Typical yield
Not determined. The yield depends on the type of bacteria and on the amount of the starting material. The typical yield is sufficient for amplification procedures.

Storage conditions:
The innuPREP Bacteria DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C! Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solutions, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-6000010 10 reactions
845-KS-6000050 50 reactions
845-KS-6000250 250 reactions
 

For isolation of viral RNA from serum, plasma or other cell-free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures.

 

Product specifications:

1. Starting material:

  • Serum, plasma, cell culture supernatants and other cell-free body fluids up to 150 µl
  • Tissue samples (max. 20 mg)
  • Swab samples
  • Paraffin embedded material
  • Cell cultures (max. 5 x 106)

2. Time for isolation:
Approximately 25 minutes

3. Positive tested for:
E.g. HIV, HCV, BVDV, Influenza

Storage conditions:
The INSTANT Virus RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Extraction Tubes, Lysis Solution, Binding Solution, Washing Solutions, RNAse-free Water, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4250010 10 reactions
845-KS-4250050 50 reactions
845-KS-4250250 250 reactions
 

For isolation of viral DNA from serum, plasma or other cell-free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures. The extraction procedure is based on a new kind of chemistry, which combines an extreme fast lysis step with a subsequent efficient binding of genomic DNA on a Spin Filter surface following washing of the bound DNA and finally eluting of the DNA.

 

product specifications:

1. Starting material:

  • Serum, plasma, cell culture supernatants and other cell-free body fluids (200 µl)
  • Tissue samples and biopsies (max. 20 mg)
  • Swab samples
  • Paraffin embedded material
  • Cell cultures (max. 5 x 106)

2. Time for isolation:
Approximately 25 minutes

3. Positive tested for:
E.g. HBV, CMV, EBV, HSV

Storage conditions:
The INSTANT Virus DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.
Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tubes, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4150010 10 reactions
845-KS-4150050 50 reactions
845-KS-4150250 250 reactions
 

For isolation of DNA from mycobacteria contained in sputum, bronchoalveolar lavage and lymph note samples. The extraction procedure combines optional a sample pre-treatment with N-actetylcysteine, pelleting of bacteria and subsequent bacteria lysis with lysozyme, following Proteinase K digestion and subsequent efficient binding of the DNA on a Spin Filter surface. The bound DNA will be washed and eluted.

 

Product specifications:

Starting material

  • Sputum from 200 µl to 5 ml)
  • Lavage up to 1 ml
  • Gewebeproben from 1 up to 10 mg

Storage conditions:
The INSTANT Mycobacteria DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K and the MAG Suspension at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tube, NAC Buffer, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No. Amount
845-KS-6150010 10 reactions
845-KS-6150050 50 reactions
845-KS-6150250 250 reactions
 

For isolation of DNA from pellets of gram  and gram  bacteria after cultivation. The extraction procedure combines a lysis step with lysozyme, following Proteinase K digestion and subsequent efficient binding of genomic DNA on a Spin Filter surface. The bound DNA will be washed and eluted.

 

Storage conditions:
The INSTANT Bacteria DNA Kit should be stored dry, at room temperature (14°C – 25 °C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Extraction Tubes, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-6050010 10 reactions
845-KS-6050050 50 reactions
845-KS-6050250 250 reactions
 

Kit for simultaneously isolation of viral DNA and RNA from different kinds of clinical specimen.

 

Product soecifications:

1. Starting material:

  • Serum, plasma, cell culture supernatants and other cell-free body fluids (150 µl)
  • Tissue samples and biopsies (max. 20 mg)
  • Swab samples
  • Paraffin embedded material
  • Cell cultures (max. 5 x 106)

2. Time for isolation:
Approximately 25 minute

Storage conditions:
The innuPREP Virus DNA/RNA Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Binding Solution, Washing Solutions, RNAse-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4800010 10 reactions
845-KS-4800050 50 reactions
845-KS-4800250 250 reactions
 

For isolation of viral RNA from serum, plasma or other cell-free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures.

 

Product specifications:

1. Starting material:

  • Serum, plasma, cell culture supernatants and other cell-free body fluids up to 150 µl
  • Tissue samples max. 20 mg
  • Swab samples
  • Paraffin embedded material
  • Cell cultures (max. 5 x 106)

2. Time for isolation:
Approximately 25 minutes

3.  Positive tested for:
E.g. HIV, HCV, BVDV, Influenza


Storage conditions:
The innuPREP Virus RNA Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Lysis Solution, Binding Solution, Washing Solution, RNase-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4700010 10 reactions
845-KS-4700050 50 reactions
845-KS-4700250 250 reactions
 

For isolation of viral DNA from serum, plasma or other cell-free body fluids, from tissue samples, paraffin embedded samples, swabs and from cell cultures. The extraction procedure is based on a new kind of chemistry, which combines an extreme fast lysis step with a subsequent efficient binding of genomic DNA on a Spin Filter surface following washing of the bound DNA and finally eluting of the DNA.

 

Product specifications:

1. Starting material:

  • Serum, plasma, cell culture supernatants and other cell-free body fluids up to 200 µl
  • Tissue samples max. 20 mg
  • Swab samples
  • Paraffin embedded material
  • Cell cultures (max. 5 x 106)

2. Time for isolation:
Approximately 25 minutes

3. Positive tested for:
E.g. HBV, CMV, EBV, HSV

Storage conditions:
The innuPREP Virus DNA Kit should be stored dry, at room temperature (14°C– 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C! Store dissolved Proteinase K at –20°C. Repeated freezing and thawing will reduce the activity dramatically.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4600010 10 reactions
845-KS-4600050 50 reactions
845-KS-4600250 250 reactions
 

For isolation of DNA from mycobacteria contained in sputum, bronchoalveolar lavage and lymph note samples. The extraction procedure combines optional a sample pre-treatment with N-actetylcysteine, pelleting of bacteria and subsequent bacteria lysis with lysozyme following Proteinase K digestion and subsequent efficient binding of the DNA on a SpinFilter surface. The bound DNA will be washed and eluted.

 

Product specification:

Starting material:

  • Sputum 200µl - 5 ml
  • Lavage 1 ml
  • Tissue 1 - 10 mg

Storage conditions:
The innuPREP Mycobacteria DNA Kit should be stored dry, at room temperature (14°C–25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.Store lyophilized Proteinase K at 4°C.
Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
NAC Buffer, Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-6100010 10 reactions
845-KS-6100050 50 reactions
845-KS-6100250 250 reactions
 

For isolation of bacterial DNA from stool samples. The kit combines a very efficient lysis of starting materials following a pre-filtration step to remove unlysed particles. After prefiltration the sample will be transferred onto a Spin Filter unit and the DNA will be bound on the Spin Filter surface. After washing of the bound DNA the DNA will be eluted from the Spin Filter and is ready to use for further downstream applications.

 

Product specifications:

1. Starting material:
Stool samples from different origins
(200 – 400 mg)

2. Time for isolation:
Approximately 30 – 45 minutes depending on the kind of application

3. Typical yield:
Not determined. Sufficient DNA for enzymatic amplification reactions.
The extracted gDNA can be used for a wide range of different molecular biology applications.

Storage conditions:
The innuPREP Stool DNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming. Store lyophilized Proteinase K at 4°C. Store dissolved Proteinase K at -20°C. Repeated freezing and thawing will reduce the activity dramatically. Dividing of the Proteinase K into aliquots and storage at -20°C is recommended.

Kit components:
Lysis Solution, Binding Solution, Proteinase K, Washing Solution, Elution Buffer, Pre-Filter, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-7000010 10 reactions
845-KS-7000050 50 reactions
845-KS-7000250 250 reactions
 

For very fast isolation of INFLUENZA RNA from primary samples of tracheal swabs or organ grid stored under medium (e.  g. enrichment medium). After sample lysis the viral RNA will be bound onto a Spin Filter surface following washing steps and finally eluting of the bound RNA.

 

Product specifications:

1. Starting material:

  • Serum, plasma, cerebrospinal fluid, liquor and cell culture supernatants (200 µl)
  • Tissue grit or tracheal swabs stored under conservation medium (for isolation of influenza viruses particular for BirdFlu testing) (200 µl of fluid sample)

2. Time for isolation:
Approximately 25 minutes

3. Positive tested for:
Different Influenza strains, including BirdFlu

Storage conditions:
The INSTANT Influenza RNA Kit should be stored dry, at room temperature (14°C – 25°C) and is stable for at least 12 months under these conditions. Before every use make sure that all components have room temperature. If there are any precipitates within the provided solutions solve these precipitates by careful warming.

Kit components:
Extraction Tubes, Lysis Solution, Washing Solutions, RNAse-free Water, Spin Filter, Receiver Tubes, Elution Tubes, Manual

Order-No.
 Amount
845-KS-4350010 10 reactions
845-KS-4350050 50 reactions
845-KS-4350250 250 reactions